Derivatives of cyclodextrins

ABSTRACT

The present invention is directed to novel aminooxy-cyclodextrin derivatives of the formula (1): CD—(X—Y—ONH 2 ) n , wherein CD is mono- or polydeoxy α-, β-, or γ-cyclodextrin, carrying in its 6-, 3- and/or 2-position a group containing the aminooxy group, and optionally carrying substituents different from (X—Y—ONH 2 ). Y is a linker group between the aminooxy group and the mono- or polydeoxy-CD group, X is a functional group or an atom necessary to connect the linker Y and the deoxy CD group, or Y is a direct bond when X is a direct bond, and n is ≧1, but ≦24, 21 and 18 for α-, β- or γ-cyclodextrin, respectively, the protected aminooxy derivatives thereof, as well the methods for their preparation and use.

This application is the national phase under 35 U.S.C. § 371 of PCT International Application No. PCT/FI99/00167 which has an International filing date of Mar. 4, 1999, which designated the United States of America.

FIELD OF THE INVENTION

This invention relates to the design and synthesis of earlier unknown chemical derivatives of cyclodextrins. The novel compounds exert a number of useful properties which make them applicable as complexants, solubilizers, carbonyl reagents, catalysts, or starting materials for the synthesis of products to be employed in pharmaceuticals, cosmetics, agriculture or in scientific laboratories.

BACKGROUND OF THE INVENTION

α-, β- and γ-Cyclodextrins (α-CD, β-CD and γ-CD) are cyclic oligosacharides consisting of 6, 7 or 8 glucopyranose units, respectively, which are joined together by α(1–4) linkages:

Cyclodextrins (termed hosts) can imbibe certain molecules or parts thereof (termed guests) into their center cavities. The noncovalent reversible adducts or inclusion complexes formed between the host and the quest can drastically change the properties of the parent guest molecules in diverse ways, such as to increase solubility, decrease volatility, protect from chemical or light-catalyzed reactions, change the location of absorption of complexed drugs in the intestine, etc.

Parent CDs can be covalently modified with a number of reagents to form chemical derivatives. The derivatives can normally bind similar guest compounds as do the parent CDs, but the properties of the complexes can be changed, A description on the syntheses of CD derivatives and the properties of inclusion complexes of both parent and modified cyclodextrins can be found for instance in Croft, A. P. & Bartsch, R. A. “Synthesis of Chemically Modified Cyclodextrins”, Tetrahedron, 1983, V. 39, No 9, P. 1417–1471 Szejtli, J. “Cyclodextrin Technology”, Kluwer Academic Publishers, Dordrecht, 1988, pp. 1–450.

Some derivatives of β-CD have a higher solubility than do the parent compound and hence they are often preferable complexants and solubilizers. The potential of the chemical derivatives of β-CD is amplified by its low price as a starting material in comparison to α- and γ-CDs. In contrast to β-CD, the more expensive α- and γ-CDs are readily water soluble and can be used without chemical derivatization for certain purposes. This is illustrated by a number of reports on their complexes with various guest compounds such as steroid hormones, cholesterol or its derivatives and with some drugs. Appropriately alkylated or hydroxyalkylated γ-CDs are also good complexants since their inclusion complexes do not precipitate even at high concentration, as stated in EP 06792.

A large number of papers deals with the syntheses of CD chemical derivatives and their application for divergent purposes (see e.g. Szejtli, J “Cyclodextrin Technology” 1988) clearly showing the importance of the CD derivatives.

SUMMARY OF THE INVENTION

The present invention describes novel CD derivatives carrying specific functions containing an aminooxy (H₂NO—) group covalently connected to a glucopyranose unit of CD. These derivatives have significantly different properties from the CD derivatives known in the prior art and thus they enlargen the area of application of CDs. The present invention also describes the preparation and use of the said novel CD derivatives as such or complexed with guest molecules or further chemically modified.

DETAILED DESCRIPTION OF THE INVENTION

The present invention is related to an earlier unknown type of α-, β- or γ-CD chemical derivatives containing the aminooxy (R₂NO—) functional group attached to the CD core and having the general formula 1: CD—(X—Y—ONH₂)_(n)  formula 1 wherein CD is mono- or polydeoxy α-, γ- or γ-CD, carrying in its 6-, 3- and/or 2-position the aminooxy function containing group, and wherein Y is a linker arm connected to deoxy-CD by means of X, which is a direct bond, or a functional group or an atom necessary to connect the linker Y and the deoxy-CD, whereby Y is a direct bond when X is a direct bond. The integer n is equal to or larger than one and cannot be more then 18, 21 and 24 for α-, β and γ-CD, respectively.

The invention also relates to the compounds of the formula 1, wherein the aminooxy group is in protected form, especially in the form of the 1-ethoxy-ethylideneaminooxy group, —O—N═C(CH₃)OC₂H₅, or as the acetone oxime group, —O—N═C(CH₃)₂.

The aminooxy-CDs of formula 1 preferably carry one or several H₂NO-groups attached to 6-hydroxy groups (see examples I–IV). By utilizing the different reactivities of primary and secondary hydroxyl groups (primary hydroxyls more reactive than secondary), and if necessary, suitably protected hydroxyl groups, one can discriminate between the reaction at the “top”, (primary) hydroxyls and at the “bottom” (secondary) hydroxyls of the CD molecule (see Croft et. al. supra). These latter types may be important for synthetizing artificial receptors, carriers and catalysts based on the CD-core.

In the compound of the formula 1, CD is a mono- or polyde-oxy α-, β- or γ-cyclodextrin. In these compounds, one or more hydroxy groups in the positions 6, 3 and/or 2 of CD are replaced with a (X—Y—ONH₂) fragment, and specifically, together with primary hydroxy groups, one or more secondary hydroxy group can also be substituted with a (X—Y—ONH₂) fragment. The compound according to the invention carrying aminooxy groups can optionally carry further substituents. In the aminooxy-CD, one or more hydroxy groups in the 6-, 3- and/or 2-position may be also substituted e.g. into H₂N—, HS—, —COOH, alkoxy, such as C₁–C₆-alkoxy, aryloxy, aryl being preferably phenyl, benzyl or tolyl, or acyloxy groups, acyl being preferably derived from C₁ –C₆-carboxylic or benzoic acid. Alkyl-, aryl- and acyloxy may carry additional functional groups in a side chain or aromatic ring.

Y is a “linker arm, or linking group” and serves as, a bridge between the aminooxy (H₂NO—) group and the deoxy-CD moiety. Usually Y is alkylene, alkenylene with one or more double bounds which may be either isolated or conjugated, alkynylene with one or more triple bonds which may be either isolated or conjugated, or arylene or arylalkylene fragments where aryl may be substituted or not substituted, such as phenylene. The alkylene, alkenylene and alkynylene fragments may be linear or branched and preferably contain 2–12 C-atoms in the chain. One or more of the chain members (methylene groups) may be replaced by —NH—, —O—, —S—, —S—S—, —C(O)NH, —C(O)O—, —OP(O)(OH)O—, —S(O)—, SO₂—, —CHR—, where R is preferably alkyl, aryl, —OR′, —NH₂, —NHR′, —NR′₂, —OH, —COOH, or —ONH₂, groups and where R′ is alkyl, aryl, or acyl. As R and R′, aryl is preferably phenyl, aryl lower alkyl, such as benzyl or tolyl.

X is preferably —O—, —S—, —NH—, —NR″—, —OCO—, —NH—O—, ═NO—, —NHC(O)—, —OP(O)(OH), —R″C═NO—, where R″ is alkyl.

R, R′ and R″ when having the meaning of alkyl, are preferably linear or branched C₁–C₆-alkyl, in the meaning of acyl they are preferably derived from linear or branched C₁–C₆-carboxylic acids or benzoic acid.

In the preferred compounds of formula 1, Y is alkylene or alkenylene of 2–12, preferably 2–6 C-atoms, wherein one or more of the chain members may be replaced by —NH—, ═N—O—, —O—, —S—, —C(O)NH—, —C(O)O—, or —CHR₁— wherein R₁ is methyl, ethyl or propyl and X is —O—, —S—, —NH—, —OC(O)— or —NH—O—.

The compounds of the formula 1 are week bases (usually the pK of the H₂NO-group is between 4.0–6.0) and their solubility is different from the parent CD molecules. As indicated by the pK values, a unique possibility exists to regulate the ionic form of the compounds of formula 1 by solvent acidity near the physiological pH-region. That means that a low pH favors complexation of ionic guest molecules, while high pH-values favor the contribution of non-ionic interactions between host and guest. With related compounds containing alkylamino functions, protonation-deprotonation takes place only at around pH 10 (Boger, J. et al Helv. Chim. Acta, 1978, V. 61, P. 2190–2218).

The compounds of formula 1 are carbonyl reagents like other O-substituted hydroxylamines. They react rapidly and quantitatively with various aldehydes and ketones forming oximes which have high stability in water solution at a broad range of pHs. These properties of aminooxy-CDs enable the synthesis of a multitude of CD derivatives in addition to those of the formula 1; for example, immobilization of CDs on solid supports and subsequent use in the chromatography of various important compounds such as stereoisomers of pharmaceuticals. In addition, oligo- and polymeric materials are readily obtained in a single-step by allowing dialdehydes or diketones to react with di- or polysubstituted aminooxy-CDs in aqueous solution. Such oligo- or polymeric materials are advantageously used as semipermeable or stereoselective membranes, as prolonged-release supports for drugs, sanitary, cosmetics or agricultural materials. Further, the chemical reactivity properties of the aminooxy functions enable one to stabilize CD-complexes of certain physiologically active, highly reactive, aldehydes and ketones—for instance, steroids, prostaglandins and vitamins—by anchoring these into CDs via the oxime bond in addition to the stabilization involved in the host-guest interaction. Since the stabilization effect is cumulative (not additive), the protection conferred by molecular complexation can be drastically increased.

Inclusion complexes in general may be additionally stabilized by means of oxime formation with a suitable aldehyde or ketone. In this case the inclusion complex is first formed which is then reacted with the aldehyde or ketone to form the inclusion complex oxime. Thus the existence of steric hindrance at the cavity entrance way prevent complex from dissociation.

While the oxime bond is stable in water solutions, especially at extreme pH values, it may slowly decompose. This property can be utilized for the slow release of aminooxy-CD bound drugs in the stomach and intestine.

Since aminooxy-CDs are carbonyl reagents, they may inhibit certain crucial enzymes in the metabolism of cells, such as PLP-, pyruvate-, or ketobutyrate-dependent enzymes. The inhibitory potency will depend on the affinity of the coenzyme to protein.

The existence of aminooxy group(s) bound to a CD molecule means that such compounds, like other O-substituted hydroxylamines, are capable of reacting directly with cytidine and adenosine. This was confirmed by the reaction of aminooxy-CDs of formula 1 with 4-thiouracil, 6-mercaptopurine riboside or their derivatives and even cytidines themself (see examples XIII and XIV). Hence, the compounds of formula 1 can be useful for the modification of nucleotides, nucleosides, bases, nucleoside coenzymes and nucleic acids, such as for the formation of nucleotide and nucleoside pyrimidine and purine derivatives of aminooxy CD, wherein the pyrimidine and purine preferably are cytosine or adenine as such, or in the form of their corresponding derivatives.

At neutral and slightly acidic pH, the aminooxy groups of compound of formula 1 are not protonated. The nonprotonated aminooxy groups are strong nucleophiles capable of reacting with an activated carboxyl group (esters, activated esters, mixed anhydrides, anhydrides, etc.) even in water solutions forming stable hydroxamic acids. These can have new useful properties such as the ability to complex certain metal ions. Combined metal ion and CD complexation functions of the aminooxy-CD derivatives may be used for recovering of metal ions from solutions.

A comparison of the compounds of the present invention with amino group containing CDs (Boger, J. et al. Helv. Chim. Acta, 1978, V. 61, P. 2190–2218) demonstrates various advantages for the aminooxy-CDs. The basic disadvantages of the alkylamino-CDs are the high pKs necessitating alkaline reaction conditions during the derivatization reactions and the low stability of the Schiff-base bond between the amino and aldehyde or keto groups in aqueous solutions.

The high nucleophilicity of the aminooxy (H₂NO—) group, Suitable compounds (3′) are e.g. 4-(ethoxyethylideneamino-oxy) bromobutene-2, ethyl N-(ω-iodoalkyloxy)acetimidate, the sodium salt of 3-(ethoxyethylideneaminooxy)-2-bromo-bromopropionic acids etc. The compounds (3′) are used in alkaline water solutions, using e.g. alkali or alkaline-earth metals, or hydrides, hydroxides, oxides, carbonates, hydrocarbonates thereof; or quaternary ammonium salts, mono-, di- and trialkylamines carrying lower (C₁ –C₄) linear or branched alkyl groups being the same or different in alkaline water-organic mixtures (the organic solvent being e.g. a lower (1–4C) alcohol, dioxane, tetrahydrofuran, glyme, cellosolve, dimethylsulfoxide, dimethylformamide) or in liquid ammonia at temperatures from elevated (about 100° C. or higher) to ambient temperature. The substitution degree depends on the reaction conditions and the products can have either only few primary hydroxyls being substituted or also secondary hydroxy groups may be involved in the reaction.

-   -   b) Alkylating an activated CD-derivative, such as a tosylate,         mesylate, halogen derivative, epoxide, activated ester, with an         aminooxy-protected, functionally substituted hydroxylamine         wherein R′₂, R′₃ and R′₄ are hydroxy, or an activated group such         as tosyl, mesyl, halogen, ester, epoxide, and X, Y and w are as         defined above, and thereafter optionally removing the protecting         group. In this reaction, aminooxy CD derivatives are obtained,         wherein X is not only O, but also e.g. sulfur or an imino group.

Suitable compounds of the formula (4) are e.g. ethyl N-(ω-mercaptoalkyloxy)-acetimidate, ethyl N-(ω-aminoalkylky-loxy) acetimidate or ethyl N-hydroxyacetimidate itself.

The activated CD-derivative can also be e.g. a mono- or poly-N-hydroxysuccinimide activated CD-derivative having a —COOH group, which is reacted with the compound of formula 4, where X is a HN-group.

According to an embodiment, one or more of the secondary hydroxy groups in the CD derivative may be unsubstituted or substituted with groups other than activating tosyl, mesyl or halogen, such as with those described above.

-   -   c) Modifying a functionally-substituted CD derivative having of         the formula (5)         wherein at least one of the groups R″₂, R″₃, and R″₄ mean         thiol-, amino- carboxy- etc. group possibly linked directly to         deoxy-CD-ring, or mean alkylenoxy- or acyloxy groups, which         contain at least one thiol-, amino-, carboxy-, etc. group, or         their derivative, and the remaining functional groups are         hydroxyl groups or hydroxyl groups at 6-, 3-, and/or         2-position(s) which are substituted with a group, for example,         H₂N—, HS—, —COOH, alkoxy-, such as C₁–C₆— alkoxy-, aryloxy-,         wherein aryl is preferably phenyl, benzyl, or tolyl, or with         acyloxy group, wherein acyl preferably originates from C₁–C₆—         carboxyl, or benzoic acids, and wherein alkyl-, aryl-, and         acyloxy- can additionally contain functional groups like H₂N—,         HS—, —COOH in their structure, in side chain or in aromatic         ring, and exist, if necessary, in a protected form, a typical         example being unsubstituted alkoxy, aryloxy, or acyloxy,         modified with an appropriate aminooxy protected substituted         hydroxylamine according to formula (3′), after which the         protecting group(s) are removed, or d) with modifying such         CD-derivative, having one or more keto or aldehyde function at         2-, 3-, and/or 6-position, optionally joined with the         above-described linkers, according to bis-aminooxyalkanes of         formula (5′)         H₂NO(CH₂)_(t)ONH₂  (5′)         wherein t=2–12 and wherein one of the methylene groups can be         replaced with O or S atoms or —NH— or —S—S— functions.

The cyclodextrin starting materials of the described reactions are well-known from literature.

Selection of a proper protecting group for aminooxy function is crucial, to be succesful in preparing the compounds of formula 1. In the present invention ethyl-N-hydroxyacetimidate fragment or alternatively acetonoxime were employed. Derivatives protected in such a way are stable in a large area of different reaction conditions and the derivatives can be readily converted to corresponding O-substituted hydroxylamines by acid treatment. In the case of ethoxylidene protection the masking group can be removed within 10–60 min at the room temperature with a diluted strong acid, exemplified by hydrohalides, sulfuric phosphoric, nitrous, and paratoluenesulfonic acids. On the contrary, removal of acetonoxime protection demands by refluxing with 20′ (w/v) hydrochloric acid.

The invention is described in the following by nonlimiting examples.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 a. Time course of the UV spectra (optical path length 1 mm) of a reaction mixture containing 1 mM 4-thiouracil in 100 mM 4-aminooxy-2-butenyl-beta-cyclodextrin (I; see Example I.2) at pH 7.0. Incubation at 20° C. is indicated as hours.

FIG. 1 b. Time course of the UV spectra (optical path length 1 mm) of a reaction mixture containing 1 mM 4-thiouracil and 100 mM 1-aminooxybutane at 20° C. at pH 7.00. Incubation time (hours) is indicated.

FIG. 2 a. Time course of the UV spectra (optical path length 1 mm) of a reaction mixture containing 1 mM 6-mercaptopurineriboside with 100 mM 4-aminooxy-2-butenyl-beta-cyclodextrin (see Example I.2). Incubation time (hours) is indicated.

FIG. 2 b. Time course of the UV spectra (optical path length b 1 mm) of a reaction mixture containing 1 mM 6-mercaptopurineriboside with 100 mM 1-aminooxybutane at pH 7.00. Incubation time (hours) is indicated.

EXAMPLE I

1. 3.1 g (14 mmoles of 1-ethoxyethylideneaminooxy-4-bromo-butene-2 (khomutov A. R. and Khomutov R. M. (1986) Bioorgan. Khim., Russ., v. 12, No. 12, p. 1662–1674) was added to a solution of 2.26 g (2 mmoles) of β-cyclodextrin in a mixture of 18.7 ml water and 1.3 ml of 10 N NaOH and heated under nitrogen on a boiling water bath with intensive stirring until the pH turned neutral (usually 60–90 min). On cooling an oil separated. The product was washed with cold water and dissolved in 50 ml of i-PrOH and 4 ml of 5.0 N, HCl was added. After a 30-minute incubation at 20° C., the liquid was decanted and the residual oil crystallized with absolute i-PrOH. The precipitation was filtered and washed with absolute i-PrOH and dried over P₂O₅/KOH in vacuo, resulting in 2.80 g (90% yield) of (I). The amount of aminooxy groups was determined (Korpela, T. K. and Makela M. J. (1981) Anal. Biochem. v.110, No. 2, p. 251–258) and was 2.8 mmols/g, the low value indicating that only primary hydroxyl groups had reacted. NMR (Jeol-400, DMSO-d₆):

2. 7.7 g (35 mmoles) of 1-ethoxyethylideneaminooxy-4-bromobutene-2 was added to a solution of 5.6 g (5 mmoles) of β-cyclodextrin in a mixture of 70 ml of water and 3.4 ml of 10 N NaOH and mixed with a magnetic stirrer at 20° C. After two days, 10 ml of i-PrOH was added and stirring continued at 20° C. until the pH turned neutral (usually about 5–8 days). The solution was evaporated to dryness in vacuo, the residual oil washed with cold water and dissolved in 50 ml of i-PrOH and 10 ml of 5.0 N HCl was added. After a 30 minute incubation at 20° C., the liquid was decanted and the residual oil crystallized with absolute i-PrOH. The precipitation was filtered and washed with absolute i-PrOH and dried over P₂O₅/KOH in vacuo, resulting in 3.56 g (50% yield) of (I). The amount of aminooxy groups determined as above was 2.26 mmols/g NMR data identical to that in ex.I.1.

EXAMPLE II

2.8 g (12 mmoles) of 1-ethoxyethylideneaminooxy-4-bromo-butene-2 was added to a solution of 1.96 g (2 mmoles) of α-cyclodextrin (Sigma) in a mixture of 18.9 ml of water and 1.1 ml of 10 N-NaOH and heated under nitrogen on a boiling water bath with intensive stirring until pH turned neutral (usually 60–90 min). The oil which separated on cooling was washed with cold water and dissolved in 50 ml i-PrOH. To this solution 4 ml of 5.0 N HCl was added. After 30 min incubation at 37° C., the liquid was decanted and the residual oil crystallized with i-PrOH. The precipitation was filtered, washed with absolute i-PrOH and dried over P₂O₅/KOH in vacuo resulting in 2.21 g (69% yield) of II. The amount of aminooxy groups determined as above was 3.25 mmoles/g. NMR (Jeol-400, DMSO-d₆):

EXAMPLE III

3.54 g (16 mmoles) of 1-ethoxyethylideneaminooxy-4-bromobutene-2 was added to a solution of 2.6 g (2 mmoles) of γ-cyclodextrin (Fluka) in a mixture of 18.5 ml of water and 1.5 ml of 10 N NaOH and heated under nitrogen on a boiling water bath with intensive stirring until the pH turned neutral (usually 60–90 min). The oil which separated on cooling was washed with cold water and dissolved in 50 ml of i-PrOH and 4 ml of 5.0 N HCl was added. After a 30-minute incubation at 20° C., the liquid was decanted and the residual oil crystallized with i-PrOH. The precipitation was filtered and washed with abs. i-PrOH and dried over P₂O₅/KOH in vacuo, resulting in 3.76 g (95% yield) of III. The amount of aminooxy groups determined as above was 2,86 mmoles/g. NMR (Jeol-400, DMSO-d₆):

EXAMPLE IV

3.1 g (14 mmoles) of 1-ethoxyethylideneaminooxybutylbromide (Nedospasov A. A and Khomutov R. M. (1976) Izv. AN SSSR Ser. Khim. (in Russian) No. 9, p. 2113–2115) was added to a solution of 2.26 g (2 mmoles) of β-cyclodextrin in a mixture of 18.7 ml water, 0.21 g (1.4 mmoles) of NaI and 1.3 ml of 10 N. NaOH and heated under nitrogen on a boiling water bath with intensive stirring until the pH turned neutral (12–18 hrs). The oil separated on chilling was washed with cold water and dissolved in 50 ml of i-PrOH. To this solution 4 ml of 5.0 N HCl was added, after 30 min incubation at 20° C. the liquid was decanted and the residual oil crystallized upon abs. i-PrOH treatment. The precipitation was filtered and washed with abs. i-PrOH and dried over P₂O₅/KOH in vacuum, that gave 1.66 g (58% yield) of IV. The amount of aminooxy groups determined as above was 2.20 mmoles/g. NMR (Jeol-400, DMSO-d₆): 10.99 (m, H₂N—O—), 4.84 (m, C₁—H), 4.02 (m, H₂NO—CH₂—), 3.76–3.38 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 1.62 (m, —CH₂—CH₂—). NMR (Jeol-400, D₂O): 4.92 (m, C₁—H), 3.95 (m, H₂NO—CH₂—), 3.71–3.45 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 1.59 (m, —CH₂—CH₂—).

EXAMPLE V

To a solution of 1.5 g of (IV) in 15 ml of H₂O-aceton mixture (1:1, V/V), diluted acqueous ammonia was added to a pH of 5–6. Then the reaction mixture was incubated for 2 h at 20° C. After evaporation to dryness, the residue was treated with water, the semi-solid product was separated and crystallized twice from water. The precipitate was filtered off, dried in vacuo over P₂O₅/KOH and 1.1 g (7% yield) of (V) was obtained. NMR (Jeol-400, DMSO-d₆): 4.83 (m, C₁—H), 3.92 (m, ═NO—CH₂—), 3.75–3.22 (m, C₃—H, C₆—H, c₅—H, C₂—H, C₄—H), 1.77 (m, (CH₃)₂C═), 1.59 (m, —CH₂—C₂—).

EXAMPLE VI

To a solution of 10.6 mg pyridoxal-5′-phosphate (“Merck”) in 1.24 ml 0.1 N NaOD in D₂O, 20 mg of (IV) was added and the reaction mixture was incubated for 2 hr. at 20° C. The compound (VI) was obtained with a yield being close to quantative. NMR (Jeol-400, D₂O): 8.39 (m, H—C═N—O—), 7.70 (m, α-H), 4.85 (m, C₁—H), 4.74 (m, —CH₂—O—P—), 4.33 (m, H₂NO—CH₂—), 3.72–3.32 (m, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 2.58 (m, α-CH₃), 1.58 (m, —CH₂—CH₂—).

EXAMPLE VII

To a solution of 0.74 g (4.5 mmoles) of 2-ethoxyethyli-deneamino-oxyethylmercaptane (Khomutov A. R. and Khomutov R. M. (1986) Bioorg. Khim. (Russ.) v. 12, No. 12, p. 1662–1674) in 2.0 ml abs. MeOH was added 2.22 ml of 2 M MeONa/MeOH, after evaporation in vacuum to dryness the residue was dissolved in a mixture of 9.5 ml abs. DMSO and 0.5 ml MeOH and added to a solution of 1.95 g (1.5 mmoles) of mono-6-O-tosyl-β-cyclodextrin (Matsui Y. and Okimoto A. (1978) Bull. Chem. Soc. (Japan) v. 51, No. 10, p. 3030–3034) in 15 ml abs. DMSO. The reaction was kept for 8 hr at 20° C., then 1.2 ml of 2 M AcOH in DMSO was added and the solution evaporated to dryness in vacuum. The residual oil solidified after water treatment, the precipitate was filtered off, washed with cold water, recrystallized twice from water and dried in vacuum over P₂O₅/KOH to give 1.3 g (yield 67%) of VII. NMR (Jeol-400, DMSO-d₆): 4.86 (m, C₁—H), 3.97 (q, CH₃—CH₂—O—), 3.94 (t, ═NO—CH₂—), 3.79–3.33 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 2.79 (m, —CH₂—CH₂—S—, 1.87 (s, CH₃—), 1.23 (t, CH₃—CH₂—O—).

EXAMPLE VIII

1.25 g (1.0 mmoles) of (VII) were suspended in 20 ml of 1 N HCl, heating to 50° C. gave clear solution, which was evaporated to dryness in vacuum. The residual oil solidified upon treatment with abs. i-PrOH The precipitate was filtered off, washed with abs. i-PrOH and dried over P₂O₅/KOH in vacuum that gave 0.80 g (65% yield) of (VIII). The amount of aminooxy groups determined as above was 0.88 mmoles/g. NMR (Jeol-400, DMSO-d₆): 4.85 (m, C₁—H) 4.12 (t, H₂NO—CH₂—), 3.82–3.33 (mm, C₃—H, C₆—HC₅—H—C₂—H, C₄—H), 2.86 (m, CH₂—CH₂—S)

EXAMPLE IX

To a solution of 0.62 g (0.5 mmoles) of (VIII) in 8.0 ml of a H₂O-acetone cocktail (1:1, V/V), a diluted water NH₃ solution was added to pH 5–6, and the reaction mixture was incubated for 2 h at 20° C. After evaporation to dryness, the residue was crystallized twice from water. The precipitate was filtered off, dried in vacuum over P₂O₅/KOH and 0.5 g (80% yield) of IX as obtained. NMR (Jeol-400, DMSO-d₆): 4.85 (m, C₁—H), 4.04 (t, ═NO—CH₂—), 3.79–3.36 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 2.77 (m, CH₂—CH₂—S), 1.80 (d, CH₃—).

EXAMPLE X

Mono-6-(4-ethoxyethylideneaminooxybutyl)thio-6-deoxy-β-cyclodextrin was obtained as is described for (VII) starting from 1.95 g (1.5 mmoles) of mono-6-O-tosyl-β-cyclodextrin and 0.85 g (4.5 mmoles) of 4-ethoxyethyli-deneaminooxyethylmercaptan (Nedospasov A. A. and Khomutov R. M. (1976) Izv. AN SSSR Ser. Khim. (in Russian) No. 9, p. 2113–2115) that gave 1.56 g (80% yield) of (X). NMR (Jeol-400, DMSO-d₆) 4.84 (m, C₁—H), 3.95 (q, CH₃—CH₂—O—), 3.80 (t, ═NO—CH₂—), 3.75–3.31 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 2.68 (m, CH₂—CH₂—S) 1.87 (d, CH₃—), 1.56 (m —CH₂—CH₂), 1.23 (t, CH₃—CH₂—O—).

EXAMPLE XI Mono-6-(4-aminooxybutyl)thio-6-deoxy-β-cyclodextrin hydrochloride (XI)

Mono-6-(4-aminooxybutyl) thio-6-deoxy-β-cyclodextrin hydrochloride was obtained as is described for (VIII) starting from 1.3 g (1.0 mmoles) of (X) that gave 1.0 g (75% yield) of (XI). NMR (Jeol-400, DMSO-d₆): 10.85 (m, H₂NO—), 4.83 (m, C₁—H), 3.99 (m, H₂NO—CH₂—), 3.63–3.31 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 1.59 (m, —CH₂—CH₂).

EXAMPLE XII

The acetonoxime of mono-6-(2-aminooxyethyl) thio-6-deox-y-β-cyclo-dextrin was obtained as is described for (IX) starting from 0.7 g of (XI) that gave 0.55 g (78% yield) of (XII). NMR (Jeol-400, DMSO-d₆): 4.82 (m, C₁—H), 3.89 (t, ═NO—CH₂—), 3.61–3.32 (mm, C₃—H, C₆—H, C₅—H, C₂—H, C₄—H), 2.92 (m, CH₂ CH₂—S), 1.78 (d, CH₃—), 1.52 (m, —CH₂—CH₂—).

EXAMPLE XIII

A 1 mM solution of 4-thiouracil (Lacheama, Brno, Czechoslovakia) was incubated at 20° C. within a 0.1 M solution of 4-aminooxy-2-butenyl-β-CD (I) at neutral pH. The UV-spectra were recorded at certain time intervals using cuvettes of 1 mm optical path length (FIG. 1 a). This reaction was compared with the reaction of 4-thiouracil with 1-aminooxybutane under the same reaction conditions (FIG. 1 b). The results show significantly higher velocity of reaction with the aminooxy-2-butenyl-β-CD.

EXAMPLE XIV

A 1 mM solution of 6-mercaptopurine riboside (Sigma Chem. Co., USA) was incubated at 20° C. within 0.1 M of 4-aminooxy-2-butenyl-β-CD (I). At certain time intervals UV-spectra were recorded in cuvettes of 1 mm optical path (FIG. 2 a) The similar reaction with 1-aminooxybutane (FIG. 2 b) showed drastically higher reaction rates with the CD-derivative. its easy introduction into different sites of the CD molecules with different spacer arms make the aminooxy-CDs and their derivatives promising for the construction of catalytically active CDs.

The compounds of formula 1 can be prepared in different ways, and the present invention is also directed to the processes for the preparation of the novel compounds of the formula 1. Such processes are:

-   a) alkylation of a corresponding CD derivative with an     aminooxy-protected, reactively substituted aminooxy derivative, for     example with a compound of the formula 3′: -    wherein R₂, R₃ and R₄ are independently hydrogen, or a substituent     (see Croft et. al. supra) having no reactive functional group, being     typically alkyl, such as C₁–C₆— alkyl, or aryl, such as phenyl,     benzyl or tolyl, whereby at least one of the positions 6, 3 and/or 2     contains a hydroxy group, preferably the 6-hydroxy group, W means     OC₂H₅ or CH₃, m and Y are as defined above and Z is a reactive     group, such as Cl, Br, I, tosyl or mesyl, and optionally removing     the protecting group. In this case a compound of the formula 1 is     obtained, wherein X is O. In the above formula, when W is OC₂H₅, the     compound (3) is protected in the form of the     1-ethoxy-ethylideneaminooxy derivative, and when W is CH₃′ in the     form of the acetone oxime derivative. 

1. Aminooxy-cyclodextrin compounds of the formula 1: CD—(X—Y—ONH₂)_(n)  (1) wherein CD is a mono- or polydeoxy α-, β- or γ-cyclodextrin, carrying in its 6-, 3- and/or 2-position the aminooxy function containing group (X—Y—ONH₂), and wherein Y is a linker group between the aminooxy group and the mono- or polydeoxy-CD-group, X is a functional group or an atom necessary to connect the linker Y and the deoxy CD group, or Y is a direct bond when X is a direct bond, and n is greater than or equal to 1, but less than or equal to 18, 21 or 24 for α-, β- or cyclodextrin, respectively.
 2. The compound according to claim 1, wherein Y and X are both direct bonds.
 3. The compound according to claim 1, wherein Y is a linear or branched alkylene, alkenylene with one or more double bonds which may be either isolated or conjugated, alkynylene with one or more triple bonds which may be either isolated or conjugated, or arylene or arylalkylene, whereby the alkylene, alkenylene and alkynylene may be linear or branched, and one or more of the chain members (methylene groups) may be replaced by —NH—, —O—, —S—, —S—S—, —C(O)NH, —C(O)O—, —OP(O)(OH)O—, —S(O)—, —SO₂—, or —CHR—, where R is alkyl, aryl, —OR′, —NH₂, —NHR′, —NR′₂, —OH, —COOH, or —ONH₂ groups and where R′ is alkyl, aryl, or acyl.
 4. The compound according to claim 1 or 3, wherein X is selected from the group consisting of —O—, —S—, —NH—, —NR″—, —OCO—, —NH—O—, ═NO—, —NHC(O)—, —OP(O)(OH)O—, and —R″C═NO—, where R″ is linear or branched lower alkyl.
 5. The compound according to claim 3, wherein Y is alkylene containing 2–12 C-atoms, wherein one or more of the chain members may be replaced by —NH—, —O—, —S—, —C(O)NH—, —C(O)O—, or CHR₁ wherein R₁ is methyl, ethyl or propyl and X is —O—, —S—, —NH—, —OC(O)—, or —NH—C(O)—.
 6. The compound according to claim 1, wherein one or more hydroxyl groups of the aminooxy cyclodextrin at 6-, 3-, and/or 2-position(s) are substituted with a group selected from the group consisting of H₂N—, HS—, —COOH, alkoxy-, aryloxy-, and acyloxy, and wherein said alkoxy-, aryloxy-, and acyloxy- can contain H₂N—, HS—, or —COOH.
 7. The aminooxy-cyclodextrin compounds of claim 1, wherein the aminooxy group is in the form of ethoxy-ethylidene or methyl-ethylidene protected aminooxy.
 8. The compounds according to claim 3, where the alkylene, alkenylene, and alkynylene contain 2 to 12 carbon atoms in the chain.
 9. The compounds of claim 6, where the alkoxy is a C₁–C₆ alkoxy, the aryloxy is phenyloxy, benzyloxy or tolyloxy, and the acyloxy originates from C₁–C₆ carboxyl or benzoic acids. 